Brown Research Trainee Steven Xu

• The goal of this training program is to acquaint the trainee with the impact of genomic features on disease predisposition and treatment resistance in
• chronic lymphocytic leukemia. The trainee will assist in isolating malignant lymphocyte populations from patient peripheral blood and bone marrow
• aspirates to continue bio-banking of primary samples to be studied in-depth for genetic mutations. Patient samples are collected from patients with
• familial association with chronic lymphocytic leukemia, and at sequential time-points on treatment with various drugs in clinical testing, including at
• the time of resistance. The trainee will also perform molecular and cell biology experiments in the laboratory that will support in vitro functional
• studies of novel genetic variants identified. The trainee will participate in characterizing novel CRISPR generated cell lines carrying each resistance
• mutation of interest. The trainee will learn how to interpret data and to design further experiments in a rationale way. Finally, the trainee will
• participate in our weekly lab meeting and then benefit from the knowledge of their colleagues.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

Learning basic clinical features of chronic lymphocytic leukemia. This will be evaluated by Dr Jennifer Brown (PI).

Understanding concepts of heritability of chronic lymphocytic leukemia. The trainee will join the Brown lab meetings to understand ongoing projects of

other lab members and provide suggestions.

Developing independence and organization skills. The trainee will be trained on project management and troubleshooting in molecular biology

research.

Learning the following techniques: Ficoll separation of mononuclear cells from peripheral blood and bone marrow aspirates; PCR; real time-qPCR;

cloning to generate genetic variants and transfection. Cell culture of lymphoid and other cancer cell lines commonly used for functional

characterization of genetic variants. Assessment of drug sensitivity (apoptosis, cell viability).

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries and when it comes to hiring the most dedicated and compassionate professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

EEO Poster

. Pay Transparency Statement

The hiring range is based on market pay structures, with individual salaries determined by factors such as business needs, market conditions, internal equity, and based on the candidate’s relevant experience, skills and qualifications.

For union positions, the pay range is determined by the Collective Bargaining Agreement (CBA).

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