Research Specialist B/C

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Posted Job Title

Research Specialist B/C

Job Profile Title

Research Specialist B

Job Description Summary

• The position will support the advancement of new therapeutics toward the clinic and contribute to the scientific work published from the lab. Its focus will be testing new engineered T cells and evaluating their functional activity, as a single agent or in combination, to enhance therapeutic T cell performance, particularly within tumor microenvironments. Both positions requires solid immunological laboratory skills, including CAR T cell manufacturing, flow cytometry, ELISA, and other in vitro assays, as well as experience with in vivo mouse studies, contributing to efforts to enhance the therapeutic index of these therapies and understand their resulting impacts within the tumor microenvironment.
• The primary function of this Research Specialist B position is to conduct in vitro and in vivo mouse studies, and assist with day-to-day operation of the research bench, all within the BRITE (Shobaki) Lab under the direction of Nour Shobaki. The purpose of this position is to support mouse studies by generating and characterizing engineered immune therapeutics, growing and developing tumor cells, performing injections, monitoring animal health, weight, and therapeutic responses, and collecting datasets from the study (tumor growth, therapeutic engraftment, histology, ex vivo assays). This position also documents and organizes experimental data and contributes to the preparation of presentations and research publications, under the guidance of the Principal Investigator.

The primary function of this Research Specialist C position is to independently design, troubleshoot, and carry out in vitro and in vivo mouse studies, maintain the animal colony, and manage day-to-day operation of the research bench, all within the BRITE (Shobaki) Lab under the direction of Nour Shobaki. The purpose of this position is to establish mouse studies by generating and characterizing engineered immune therapeutics, growing and developing tumor cells, performing injections, monitoring animal health, weight, and therapeutic responses, and collecting datasets from the study (tumor growth, therapeutic engraftment, histology, ex vivo assays). This position also generates and organizes the experimental data needed to support grant applications and IND-enabling studies, and contributes written sections to progress reports and manuscripts, with final authorship and submission led by the Principal Investigator.

Job Description

Job Responsibilities Research Specialist B

Conduct in vitro immunological functional assays, including CAR T cell manufacturing, flow cytometry, and ELISA, following established protocols; troubleshoot experimental issues as they arise

Conduct in vivo mouse studies, executing established protocols under the direction of the PI

Prepare tumor cells and engineered immune therapies for in vivo experiments, documenting their characteristics and performing assays to assess functional performance

Monitor and collect datasets associated with animal studies, including health monitoring and weights, tumor growth (caliper, bioimaging), bleeds, and necropsies. Perform IV, IT, IP, and SC injections of tumor cells and therapeutics.

Prepare tissues at necropsy by fixing and isolating cells for functional and flow cytometric analysis, IHC, and spatial transcriptomic studies.

Document experimental data, perform data analysis, and contribute to the preparation of presentations and research publications under the guidance of the Principal Investigator.

Participate in lab management and support lab set-up and operations, including ordering, organizing, and maintaining reagents, equipment, and protocols

Characterize transgenic mice and maintain colony.

Train and provide guidance to other lab members as needed.

Job Responsibilities Research Specialist C

Independently design, troubleshoot, and perform in vitro immunological functional assays, including CAR T cell manufacturing, flow cytometry, and ELISA; contribute to development of new methods using state-of-the-art techniques.

Independently design, troubleshoot, and execute in vivo mouse studies using established protocols, and contribute to development of new protocols under the direction of the lab supervisor

Modify and prepare tumor cells and engineered immune therapies for in vivo mouse experiments, documenting their characteristics and performing assays defining functional performance.

Monitor and collect datasets associated with animal studies, including health monitoring and weights, tumor growth (caliper, bioimaging), bleeds, and necropsies. Perform IV, IT, IP, and SC injections of tumor cells and therapeutics

Prepare tissues at necropsy by fixing and isolating cells for functional and flow cytometric analysis, IHC, and spatial transcriptomic studies.

Document experimental data, perform data analysis, generate and organize the data needed to support grant applications and IND-enabling studies, and write sections of manuscripts and progress reports describing research results.

Provide scientific and technical guidance and mentorship to other lab members and trainees, contributing to research team performance.

Manage day-to-day research bench operations, including maintaining general lab supplies and reagent inventory, equipment upkeep, and protocol organization

Characterize transgenic mice and maintain colony.

Position is funded for one year with the possibility of being extended.

Qualifications

Required:

Research Specialist B:

• •Bachelor's degree in Immunology, Cell Biology, Biomedical Sciences, or a related field, with 1–2 years of relevant research experience, or an equivalent combination of education and experience
• •Hands-on experience with in vitro immunological assays, including flow cytometry, ELISA, and cell culture
• •Exposure to in vivo mouse models, including tumor inoculation, therapeutic administration, or monitoring
• •Strong organizational and documentation skills

Research Specialist C:

• •Bachelor's degree in Immunology, Cell Biology, Biomedical Sciences, or a related field, with 3–5 years of relevant research experience, or an equivalent combination of education and experience
• •Hands-on experience with in vitro immunological assays, including flow cytometry, ELISA, cell culture, and co-culture killing assays
• •Experience with in vivo mouse models, including tumor inoculation, therapeutic administration, and monitoring
• •Experience with immunotherapy
• •Strong organizat